5IIL

Crystal structure of the post-catalytic nick complex of DNA polymerase lambda with a templating 8-oxo-dG and incorporated dA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-13
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	56.220, 63.554, 139.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.109 - 1.960
R-factor	0.1809
Rwork	0.179
R-free	0.21720
RMSD bond length	0.007
RMSD bond angle	1.015
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	69.920
High resolution limit [Å]	1.960
Number of reflections	35462
<I/σ(I)>	24.2
Completeness [%]	96.2
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.2 M ammonium acetate, 0.1 M sodium citrate pH 4.8, and 3-5% PEG 4000