5IIK

Crystal structure of the post-catalytic nick complex of DNA polymerase lambda with a templating 8-oxo-dG and incorporated dC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	56.182, 63.475, 140.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.070 - 1.982
R-factor	0.1939
Rwork	0.192
R-free	0.23780
RMSD bond length	0.007
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.840
High resolution limit [Å]	1.980
Number of reflections	32242
<I/σ(I)>	31.2
Completeness [%]	92.3
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.2 M ammonium acetate, 0.1 M sodium citrate pH 4.8, and 2-7% PEG 4000