5IIJ

Crystal structure of the pre-catalytic ternary complex of DNA polymerase lambda with a templating 8-oxo-dG and an incoming dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	55.749, 62.850, 140.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.706 - 1.724
R-factor	0.1982
Rwork	0.197
R-free	0.22400
RMSD bond length	0.007
RMSD bond angle	1.071
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	70.038
High resolution limit [Å]	1.724
Number of reflections	52702
<I/σ(I)>	22.8
Completeness [%]	100.0
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M sodium cacodylate pH 7.0 and 1.0 M sodium acetate trihydrate