5II6
Crystal structure of the ZP-N1 domain of mouse sperm receptor ZP2 at 0.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.539, 50.013, 51.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.419 - 0.950 |
| R-factor | 0.1276 |
| Rwork | 0.127 |
| R-free | 0.14480 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.458 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.419 | 0.984 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.022 | 0.242 |
| Number of reflections | 64506 | |
| <I/σ(I)> | 13 | 2.66 |
| Completeness [%] | 99.0 | 90 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 200, 5% PEG 3000, 0.1 M MES |
