5IHW
The crystal structure of SdrE from staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2013-04-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9782 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.260, 41.560, 51.140 |
| Unit cell angles | 95.59, 97.55, 99.55 |
Refinement procedure
| Resolution | 50.320 - 1.250 |
| R-factor | 0.1444 |
| Rwork | 0.143 |
| R-free | 0.17640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cf3 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.263 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.320 |
| High resolution limit [Å] | 1.250 |
| Rmerge | 0.056 |
| Number of reflections | 75580 |
| <I/σ(I)> | 8.1 |
| Completeness [%] | 87.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2mol/L CaCl2, 35% PEG 3350, 0.1mol/L, Bis-Tris pH 6.6 |
