5IHU
Crystal structure of bovine Fab B11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.410, 71.460, 88.320 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | 38.890 - 2.060 |
| R-factor | 0.1975 |
| Rwork | 0.194 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3d |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.850 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.050 | 0.970 |
| Number of reflections | 28518 | |
| <I/σ(I)> | 13.8 | 1.6 |
| Completeness [%] | 95.0 | 94.1 |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 5% PEG 3000 30% PEG 400 10% glycerol 0.1M HEPES |
