5IHF
Salmonella Typhimurium VirG-like (STV) protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.960, 59.710, 63.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.380 - 1.576 |
| R-factor | 0.2035 |
| Rwork | 0.203 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5io8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.785 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.130 | 43.380 | 1.660 |
| High resolution limit [Å] | 1.576 | 4.980 | 1.580 |
| Rmerge | 0.052 | 0.680 | |
| Number of reflections | 30511 | ||
| <I/σ(I)> | 9.7 | 10.5 | 1.2 |
| Completeness [%] | 97.6 | 83.8 | 98.5 |
| Redundancy | 3.8 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.8 M Sodium Phosphate Monobasic Monohydrate, Potassium Phosphate Dibasic |
