5IHB

Structure of the immune receptor CD33

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	64.960, 127.040, 143.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.320 - 2.240
R-factor	0.2003
Rwork	0.198
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Model derived from 2ZG2 by Sculptor
RMSD bond length	0.008
RMSD bond angle	0.779
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2313))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.320	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.070	0.785
Number of reflections	56518
<I/σ(I)>	11.92	1.9
Completeness [%]	98.1	99
Redundancy	4.7	4.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295.15	2% PEG 20,000, 4% PEG MME 500, 100 mM Bicine/Tris base pH 8.5, 1xMorpheus amino acids