5IH5

Human Casein Kinase 1 isoform delta (kinase domain) in complex with Epiblastin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92019
Spacegroup name	P 31 2 1
Unit cell lengths	65.070, 65.070, 151.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.210 - 2.250
R-factor	0.241
Rwork	0.240
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih4
RMSD bond length	0.019
RMSD bond angle	1.836
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.210		2.310
High resolution limit [Å]	2.250	10.070	2.250
Rmerge	0.186	0.034	1.297
Rmeas	0.192	0.036	1.361
Total number of observations	338713
Number of reflections	17788	249	838
<I/σ(I)>	13.19	62.18	1.59
Completeness [%]	97.5	98.4	64.8
Redundancy	19		9.7
CC(1/2)	0.999	0.999	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Li2SO4, 0.7 - 0.8 M Na-K tartrate, 0.1 M CHES