5IGZ

Macrolide 2'-phosphotransferase type II - complex with GDP and spiramycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-23
Detector	RAYONIX MX-300
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	55.240, 72.430, 77.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.900 - 1.600
R-factor	0.1623
Rwork	0.161
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5igv
RMSD bond length	0.019
RMSD bond angle	1.479
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.900		1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.049	0.020	0.675
Number of reflections	41503
<I/σ(I)>	23.59	66.49	3.03
Completeness [%]	100.0	99.3	100
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	0.1 M calcium acetate, 0.1M Tris, 25-40% PEG 4000