5IGV

Macrolide 2'-phosphotransferase type II - complex with GDP and azithromycin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-23
Detector	RAYONIX MX-300
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	40.230, 80.450, 96.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.360 - 1.550
R-factor	0.179
Rwork	0.178
R-free	0.20900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5iwu
RMSD bond length	0.009
RMSD bond angle	1.128
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10PRE_2104)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.356	1.590
High resolution limit [Å]	1.550	1.550
Rmerge	0.038	0.640
Number of reflections	46230
<I/σ(I)>	30.01	3.59
Completeness [%]	100.0	100
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	0.1 M calcium acetate, 0.1M Tris, 25-40% PEG 4000