5IGO
WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.516, 87.469, 94.610 |
| Unit cell angles | 90.00, 92.55, 90.00 |
Refinement procedure
| Resolution | 64.200 - 1.600 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.21340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hqg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.438 | 1.600 |
| High resolution limit [Å] | 1.597 | 1.597 |
| Rmerge | 1.160 | |
| Number of reflections | 167321 | |
| <I/σ(I)> | 9 | 0.92 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1M Tris 8.5, 16% PEG3350, 2% Tacsimate 8.0. |
