5IG5
Crystal structure of N. vectensis CaMKII-B hub at pH 4.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.974, 113.974, 241.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.352 - 3.000 |
| R-factor | 0.2342 |
| Rwork | 0.232 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hkx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.625 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2229: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.352 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.134 | 1.373 |
| Number of reflections | 37190 | |
| <I/σ(I)> | 11.1 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 2.5 M sodium chloride, 0.1 M acetate pH 4.2, and 0.25 M lithium sulphate |
