5IG0
Crystal structure of S. rosetta CaMKII hub
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 96.805, 96.805, 58.416 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.918 - 1.750 |
| R-factor | 0.1923 |
| Rwork | 0.191 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hkx |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.043 | 0.783 |
| Number of reflections | 16674 | |
| <I/σ(I)> | 22.2 | 1.7 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M lithium sulphate, 0.1 M Tris pH 7.0, 2.0 M ammonium sulphate |
