5IFP

STRUCTURE OF BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97948
Spacegroup name	P 21 21 21
Unit cell lengths	85.570, 111.421, 126.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.680 - 1.710
R-factor	0.15127
Rwork	0.150
R-free	0.17136
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iug
RMSD bond length	0.006
RMSD bond angle	1.272
Data reduction software	MOSFLM (7.0.9)
Data scaling software	Aimless (0.5.21)
Phasing software	MOLREP (11.4.04)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.680	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.115	0.599
Number of reflections	131079
<I/σ(I)>	11.5	3.7
Completeness [%]	99.9	99.9
Redundancy	6.8	6.2
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	21% (W/V) PEG 3350, 0.1M BIS-TRIS BUFFER PH 5.5 , 0.2M LITHIUM SULPHATE