5IFN
Human PSPC1 Homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.536, 63.493, 67.800 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 19.410 - 3.170 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sde |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.410 |
| High resolution limit [Å] | 3.170 |
| Number of reflections | 8738 |
| <I/σ(I)> | 5.7 |
| Completeness [%] | 97.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM (D/L) malic acid pH 7.0 28% PEG 3350 100 mM Tris pH 7.0 |
