5IFG
Crystal structure of HigA-HigB complex from E. Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.924, 77.924, 180.634 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.928 - 2.702 |
| R-factor | 0.2019 |
| Rwork | 0.199 |
| R-free | 0.23080 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.668 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.105 | 0.519 |
| Number of reflections | 35954 | |
| <I/σ(I)> | 65.78 | 14.67 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 17.4 | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.2M (NH4)2SO4, 0.1M Bis-Tris pH5.3, 20%(w/v) PEG3350, 0.01M KCl |
