5IF1
Crystal structure apo CDK2/cyclin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 186.574, 186.574, 213.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.070 - 2.610 |
| R-factor | 0.18986 |
| Rwork | 0.187 |
| R-free | 0.23632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.070 |
| High resolution limit [Å] | 2.610 |
| Number of reflections | 63451 |
| <I/σ(I)> | 11.4 |
| Completeness [%] | 100.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.15 | 100mM HEPES, 5mM DTT, 0.5 mM Kcl, 875mM ammonium sulfate |
