5IEL
Structure of Lysozyme labeled with fluorescein isothiocyanate (FITC) at 1.15 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97241 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.756, 80.756, 36.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.100 - 1.150 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ngi |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.265 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.100 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.050 | 0.400 |
| Number of reflections | 43530 | |
| <I/σ(I)> | 16.5 | 1.76 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 | 0.1M HEPES 30% v/v Jeffamine ED-2001 ; 0.2M Magnesium nitrate hexahydrate 20% w/v Polyethylene glycol 3,350 |
