5IEK

Structure of HLA-B*40:02 in complex with the endogenous peptide REFSKEPEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00556
Spacegroup name	P 21 21 21
Unit cell lengths	51.260, 82.201, 111.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.975 - 1.800
R-factor	0.163
Rwork	0.162
R-free	0.18810
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2148)

Data quality characteristics

	Overall
Number of reflections	46622
<I/σ(I)>	10.8
Completeness [%]	99.9
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	150 mM Tris 8.5, 23% PEG8K, 70 mM potassium thiocyanate