5IE9
Crystal structure of the Bacillus-conserved MazG protein, a nucleotide pyrophosphohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2014-05-11 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 32 |
| Unit cell lengths | 90.578, 90.578, 97.056 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.2232 |
| Rwork | 0.222 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gta |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.800 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.7 | 89.4 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1mM sodium cacodylate pH 6.5, 1M sodium citrate |
