5IE0
Crystal structure of a plant enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.164, 117.763, 72.160 |
| Unit cell angles | 90.00, 93.71, 90.00 |
Refinement procedure
| Resolution | 28.586 - 2.000 |
| R-factor | 0.1741 |
| Rwork | 0.173 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a9v |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.486 |
| Number of reflections | 80270 | |
| <I/σ(I)> | 10.8 | 3.2 |
| Completeness [%] | 99.3 | 93.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 281 | 0.2M ammonium tartrate dibasic, 20% PEG 3350 |
