5IDO

RNA Editing TUTase 1 from Trypanosoma brucei in complex with UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 21 2 21
Unit cell lengths	59.350, 66.340, 128.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.727 - 2.200
R-factor	0.2498
Rwork	0.248
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hzd
RMSD bond length	0.003
RMSD bond angle	0.778
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.720		2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.071	0.032	0.654
Number of reflections	26453
<I/σ(I)>	22.88	53.95	3.91
Completeness [%]	99.4	82	99.8
Redundancy	12.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1M Tris pH8.5, between 5% and 8% PEG 3350, 0.2M Lithium chloride.Complex of D473A with UTP was obtained by soaking 1.5mM UTP supplemented with 1mM MgCl2