5ICU
The crystal structure of CopC from Methylosinus trichosporium OB3b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-04-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.671, 54.125, 65.371 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.980 - 1.460 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.19112 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.665 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.980 |
| High resolution limit [Å] | 1.460 |
| Number of reflections | 17434 |
| <I/σ(I)> | 41.9 |
| Completeness [%] | 99.9 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M HEPES sodium salt, 0.8 M sodium phosphate, and 0.8 M potassium phosphate, pH 7.5. |
