5IBW
Complex of MlcC bound to the tandem IQ motif of MyoC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.680, 31.410, 56.410 |
| Unit cell angles | 75.79, 84.00, 67.06 |
Refinement procedure
| Resolution | 28.200 - 1.900 |
| Rwork | 0.140 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ix7 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | |
| Number of reflections | 12613 | |
| <I/σ(I)> | 26.9 | |
| Completeness [%] | 90.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.7M NaH2PO4/K2HPO4 pH 5.6 |
