5IB9
Crystal structure of aminopeptidase equipped with PAD from Aneurinibacillus sp. AM-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2012-05-16 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.180, 68.443, 76.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.800 - 1.400 |
| R-factor | 0.18085 |
| Rwork | 0.179 |
| R-free | 0.22284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ek9 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.268 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 89717 |
| <I/σ(I)> | 27.6 |
| Completeness [%] | 93.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.8 | 293 | 13% PEG 6000, 0.2M Zinc acetate, 0.1M MES-NaOH, pH 5.8 |
