5IB8

Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNALys with U-G mismatch in the A-site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	209.150, 448.160, 617.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	154.450 - 3.130
R-factor	0.1921
Rwork	0.192
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e7k
RMSD bond length	0.009
RMSD bond angle	1.491
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	154.450		3.210
High resolution limit [Å]	3.130	14.000	3.130
Rmerge	0.419	0.070	4.322
Number of reflections	1008570
<I/σ(I)>	42.1	89.7	0.97
Completeness [%]	100.0	99.7	100
Redundancy	133.67
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 20000, PEG 550mme, KSCN, Tris-OAc