5IAA
Crystal structure of human UBA5 in complex with UFM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.896 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 138.081, 138.081, 99.178 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.040 - 1.850 |
| R-factor | 0.18834 |
| Rwork | 0.187 |
| R-free | 0.20605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WXS and 3H8V |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.766 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.300 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.174 | |
| Number of reflections | 93040 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 18.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 8% Tascimate, pH 7.0 and 16% PEG3350 |
