5IA3

Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with PD173955

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.85506
Spacegroup name	P 1 21 1
Unit cell lengths	32.766, 107.543, 40.534
Unit cell angles	90.00, 108.68, 90.00

Refinement procedure

Resolution	53.772 - 1.788
R-factor	0.1629
Rwork	0.161
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mqb
RMSD bond length	0.006
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.772	1.794
High resolution limit [Å]	1.788	1.788
Rmerge	0.139	0.960
Number of reflections	24769
<I/σ(I)>	9.51
Completeness [%]	98.0
Redundancy	3.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	37.5 % MPD_P1k_P3350, 0.2 M Amino Acids, 0.1 M Buffer3 pH 8.5