5I9U
Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.715, 104.904, 39.681 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 27.712 - 1.889 |
| R-factor | 0.1661 |
| Rwork | 0.163 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mqb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.855 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.590 | 1.990 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rpim | 0.134 | 0.603 |
| Number of reflections | 20489 | |
| <I/σ(I)> | 7.46 | |
| Completeness [%] | 94.4 | |
| Redundancy | 2.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 37.5 % MPD_P1k_P3350, 0.1 M Amino Acids, 0.1 M Bicine/Trizma base pH 8.5 |
