5I9O
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K L103K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-01-29 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.195, 78.933, 60.891 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 60.890 - 1.950 |
| R-factor | 0.2015 |
| Rwork | 0.200 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.806 |
| Data reduction software | XPREP |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.890 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.047 | 0.216 |
| Number of reflections | 21550 | |
| <I/σ(I)> | 28.05 | 4.46 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
