5I9G

Crystal structure of designed pentatricopeptide repeat protein dPPR-U8C2 in complex with its target RNA U8C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9806
Spacegroup name	P 21 21 21
Unit cell lengths	51.433, 84.782, 94.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.668 - 2.288
R-factor	0.2261
Rwork	0.225
R-free	0.24750
RMSD bond length	0.016
RMSD bond angle	1.802
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.280
Number of reflections	18810
<I/σ(I)>	26.3
Completeness [%]	98.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 3350, Bis-Tris propane, Magnesium chloride, Ethyl Acetate, D-(+)-glucose monohydrate