5I8Q

S. cerevisiae Prp43 in complex with RNA and ADPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	168.063, 154.031, 78.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.446 - 4.200
R-factor	0.2629
Rwork	0.260
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kx2
RMSD bond length	0.002
RMSD bond angle	0.567
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		4.340
High resolution limit [Å]	4.200	9.250	4.200
Rmerge	0.255	0.038	1.054
Number of reflections	15735
<I/σ(I)>	8.67	34.24	2.08
Completeness [%]	99.5	95.7	99.9
Redundancy	6.05
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		292	Crystallization buffer was prepared by mixing a solution containing 100 mM MES-NaOH, pH 6.0, 100 mM NaOAc, 8% PEG 10K and an additive solution (20% acetonitrile, 5% n-Dodecyl-?-D-maltoside) in a 5:1 (v:v).Microbatch drops were formed by mixing 1.8 ?l of the crystallization buffer with 1.2 ?l of the preformed complex