5I8L
Crystal structure of the full-length cell wall-binding module of Cpl7 mutant R223A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.05793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.524, 50.331, 86.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.873 - 2.801 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.28910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cvd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.040 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.150 |
| Number of reflections | 2800 | |
| <I/σ(I)> | 10.8 | 6.3 |
| Completeness [%] | 81.2 | 90.8 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.1 M tri-Sodium citrate |
