5I8K
HHH1 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.647, 62.647, 227.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.331 - 1.771 |
| R-factor | 0.1842 |
| Rwork | 0.183 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4MA3 with CDR regions removed |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.982 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.331 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 45033 | |
| <I/σ(I)> | 21.15 | |
| Completeness [%] | 99.2 | |
| Redundancy | 10.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15-19% (v/v) Isopropanol, 15-24.5% (w/v) PEG 3350, 0.075 M MES pH 5.5-6.0 |
