5I84
Structure of the Xanthomonas citri phosphate-binding protein PhoX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-12-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.459 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.556, 115.643, 133.323 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 17.060 - 2.980 |
| R-factor | 0.17804 |
| Rwork | 0.174 |
| R-free | 0.24922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abh |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.173 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.360 | 3.110 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Number of reflections | 46810 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 94.3 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 200 mM sodium iodide 20% PEG 3350 |
