5I7E
Crystal structure of B. pseudomallei FabI in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.849999 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.686, 75.243, 85.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.325 - 1.650 |
| R-factor | 0.1599 |
| Rwork | 0.159 |
| R-free | 0.18610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.042 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.620 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.061 | 0.702 |
| Number of reflections | 28203 | |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 96.6 | 98.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES, pH 6.5, 30 % PEG 400 |
