5I7D
Crystal Structure of srGAP2 F-BARx WT Form-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.008818 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.904, 49.926, 234.084 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 46.390 - 3.950 |
| R-factor | 0.28037 |
| Rwork | 0.277 |
| R-free | 0.34941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.950 |
| Number of reflections | 12297 |
| <I/σ(I)> | 8.32 |
| Completeness [%] | 98.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | F-BARx eluted in 5% Glycerol, 25 mM Tris buffer pH=8.5, 1 mM DTT, and ~0.2 M NaCl at the elution peak, concentrated to ~15 mg/ml and crystallized in 0.2 M Ammonium citrate tribasic pH 6.5, 0.2 M Guanidine, 0.3 M NaCl, 16% PEG 3350 |
