5I7C
Centrosomin-motif 2 (CM2) domain of Drosophila melanogaster Centrosomin (Cnn)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 |
| Unit cell lengths | 49.220, 49.220, 211.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.626 - 2.804 |
| R-factor | 0.2439 |
| Rwork | 0.242 |
| R-free | 0.26760 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.555 |
| Data reduction software | xia2 (0.3.6.3) |
| Phasing software | AutoSol (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.626 | 2.880 |
| High resolution limit [Å] | 2.804 | 2.804 |
| Rmerge | 0.069 | |
| Number of reflections | 7171 | |
| <I/σ(I)> | 19.7 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 10.1 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 80 mM Sodium cacodylate pH6.5, 160 mM Calcium acetate, 14.4% w/v PEG8K/ 20% v/v glycerol |
