5I70
Crystal Structure of plasmepsin IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-08 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.420, 103.420, 123.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 3.000 |
| R-factor | 0.25122 |
| Rwork | 0.248 |
| R-free | 0.29421 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.671 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.110 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 15066 |
| <I/σ(I)> | 10 |
| Completeness [%] | 95.3 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.10M sodium phosphate buffer (pH 5.5) and 60% (NH4)2SO4 |
