5I6R
Crystal Structure of srGAP2 F-BARx WT Form-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 93.300, 29.760, 94.630 |
| Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
| Resolution | 94.500 - 2.150 |
| R-factor | 0.20562 |
| Rwork | 0.203 |
| R-free | 0.26013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 94.500 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 28948 |
| <I/σ(I)> | 11.12 |
| Completeness [%] | 99.4 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | F-BARx-C280Y was eluted in 25 mM phosphate buffer pH 8, 120 mM NaCl, 1 mM DTT, concentrated to 12 mg/ml, and crystallized in 5% v/v Tacsimate pH=5.5, 12% w/v PEG 3350. |
