Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.346, 32.134, 34.296 |
| Unit cell angles | 88.04, 71.17, 68.35 |
Refinement procedure
| Resolution | 32.310 - 1.420 |
| R-factor | 0.18734 |
| Rwork | 0.185 |
| R-free | 0.23687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mwn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.894 |
| Data reduction software | SAINT |
| Data scaling software | APEX |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.310 | 1.510 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.145 | 0.554 |
| Number of reflections | 18176 | |
| <I/σ(I)> | 6.8 | 1.1 |
| Completeness [%] | 94.8 | 98.5 |
| Redundancy | 5.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.7 | 294 | CRYSTALLIZATION CONDITIONS: 40MG HEWL CO-CRYSTALLISED WITH 2.6MG CISPLATIN, WITH THE PLATINUM COMPOUNDS BEING IN A 3-FOLD MOLAR EXCESS TO THE PROTEIN, 462.5 UL OF A 0.02M NAAC SOLUTION ALONG WITH 462.5 UL OF A 0.5M NANO3 SOLUTION WAS USED WITH 75 UL DMSO ADDED, BATCH, PH 4.7, EVAPORATION, TEMPERATURE 294K |
