5I5O
Crystal Structure of N-terminal Domain of Matrix Protein of Thogoto Virus at Neutral pH.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.037, 73.298, 94.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.156 - 2.682 |
| R-factor | 0.2726 |
| Rwork | 0.271 |
| R-free | 0.28520 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.156 | 2.750 |
| High resolution limit [Å] | 2.682 | 2.680 |
| Rmerge | 0.135 | 0.589 |
| Number of reflections | 7381 | |
| <I/σ(I)> | 12.93 | 4.17 |
| Completeness [%] | 97.5 | 100 |
| Redundancy | 10.1 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M Ammonium citrate tribasic pH 7.0, 20%(w/v) Polyethylene glycol 3350 |
