5I5N
Crystal Structure of N-terminal Domain of Matrix Protein of Thogoto Virus at Acidic pH.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.982, 88.353, 88.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.510 - 1.418 |
| R-factor | 0.1766 |
| Rwork | 0.176 |
| R-free | 0.18980 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.640 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.510 | 1.440 |
| High resolution limit [Å] | 1.418 | 1.420 |
| Rmerge | 0.078 | 0.517 |
| Number of reflections | 55605 | |
| <I/σ(I)> | 26.7 | 4.65 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 0.1M Sodium acetate trihydrate pH 4.5, 3.0M NaCl |
