5I4L
Crystal structure of Amicoumacin A bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 434.230, 287.910, 304.120 |
| Unit cell angles | 90.00, 99.11, 90.00 |
Refinement procedure
| Resolution | 103.619 - 3.100 |
| R-factor | 0.2132 |
| Rwork | 0.212 |
| R-free | 0.25140 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 103.620 | 3.200 | |
| High resolution limit [Å] | 3.100 | 105.000 | 3.100 |
| Rmerge | 0.225 | 1.562 | |
| Number of reflections | 1330001 | ||
| <I/σ(I)> | 6.92 | 1.02 | |
| Completeness [%] | 100.0 | 100 | |
| Redundancy | 6.64 | ||
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
