5I4K
Metal ABC transporter from Listeria monocytogenes with manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.906, 62.311, 75.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.400 - 1.790 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18910 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.424 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.400 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.790 | 4.880 | 1.790 |
| Rmerge | 0.111 | 0.092 | 0.872 |
| Number of reflections | 25812 | ||
| <I/σ(I)> | 9.9 | 2.25 | |
| Completeness [%] | 99.6 | 96.4 | 100 |
| Redundancy | 5.9 | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 25% PEG 8000, 0.2 M manganese chloride, 0.1 M Tris |
