5I3L
DPF3b in complex with H3K14ac peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28215 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.822, 121.641, 56.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.850 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.21170 |
| Starting model (for MR) | unpublished model ultimately derived from PDB entry 3V43. |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.102 | 1.125 |
| Rmeas | 0.110 | 1.214 |
| Rpim | 0.041 | 0.453 |
| Total number of observations | 210147 | 12925 |
| Number of reflections | 29753 | |
| <I/σ(I)> | 17.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.1 |
| CC(1/2) | 0.998 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.4 M sodium citrate, 0.1 M HEPES, 5% MPD |
