5I2Q

Structure of EF-hand containing protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	95
Detector technology	CCD
Collection date	2014-04-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	35.598, 52.107, 55.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.394 - 1.935
R-factor	0.1735
Rwork	0.172
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i2l
RMSD bond length	0.009
RMSD bond angle	1.020
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.047	0.271
Number of reflections	7948
<I/σ(I)>	15.4	6.1
Completeness [%]	98.8	98.7
Redundancy	9.3	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.1 M Tris-HCl, pH8.5, 23 % PEG 2000