5I2O

Structure of EF-hand containing protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	95
Detector technology	CCD
Collection date	2014-04-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	36.254, 51.529, 53.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.136 - 1.952
R-factor	0.1846
Rwork	0.183
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i2l
RMSD bond length	0.013
RMSD bond angle	1.365
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.062	0.383
Number of reflections	7706
<I/σ(I)>	9.8
Completeness [%]	98.7	78.7
Redundancy	10.6	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.1M Tris-HCl, pH8.5, 32% PEG 2000