5I2L

Structure of EF-hand containing protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	95
Detector technology	CCD
Collection date	2009-04-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796, 0.9793, 0.9716, 0.9873,1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	37.325, 50.670, 53.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.750 - 1.850
R-factor	0.1667
Rwork	0.162
R-free	0.20680
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.150
Phasing software	PHENIX (1.7.2_869)
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.083	0.412
Number of reflections	8999
<I/σ(I)>	13.1	9.9
Completeness [%]	99.3	97.3
Redundancy	9.4	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	23% PEG 4000, 0.16M Na-acetate, 3% dioxane, 0.1 M Tris-HCl, pH 8.5